Supplemental Information 2-Fluoro Substituents Can Mimic Native 2-Hydroxyls within Structured RNA
نویسندگان
چکیده
TABLE S1, related to Table 1. Binding and reactivity of AUCG with closed complexes of different ribozymes, using the-1d,rSA or the-1d,rSA 5 substrates. Numbers in parentheses represent the values relative to the A261OH ribozyme.
منابع مشابه
2'-Fluoro substituents can mimic native 2'-hydroxyls within structured RNA.
The ability of fluorine in a C-F bond to act as a hydrogen bond acceptor is controversial. To test such ability in complex RNA macromolecules, we have replaced native 2'-OH groups with 2'-F and 2'-H groups in two related systems, the Tetrahymena group I ribozyme and the ΔC209 P4-P6 RNA domain. In three cases the introduced 2'-F mimics the native 2'-OH group, suggesting that the fluorine atom ca...
متن کامل20-Fluoro Substituents Can Mimic Native 20-Hydroxyls within Structured RNA
Marcello Forconi,1,3,6 Jason P. Schwans,1,4,6 Rishi H. Porecha,1 Raghuvir N. Sengupta,1,4 Joseph A. Piccirilli,4,5,* and Daniel Herschlag1,2,* 1Department of Biochemistry 2Department of Chemistry Stanford University, Stanford, CA 94305, USA 3Department of Chemistry and Biochemistry, College of Charleston, Charleston, SC 29424, USA 4Department of Chemistry 5Department of Biochemistry and Molecul...
متن کاملSite specific deoxynucleotide substitutions in yeast U6 snRNA block splicing of pre-mRNA in vitro.
We have identified 2'-hydroxyl groups of the U6 phosphate-ribose backbone which are required for reconstitution of splicing activity in U6-depleted yeast extract. To screen the 2'-hydroxyls of yeast U6 at nucleotides 39-88, spanning the conserved central domain, synthetic U6 RNAs were constructed with deoxyribonucleotides incorporated site specifically. Only four individual deoxynucleotide subs...
متن کاملSynthesis and microwave assisted polymerization of fluorinated 2-phenyl-2-oxazolines: the fastest 2-oxazoline monomer to date.
The living cationic ring-opening polymerization of 2-oxazolines with fluorinated aromatic substituents was found to be strongly accelerated by o-fluoro substituents.
متن کاملProbing the folding landscape of the Tetrahymena ribozyme: commitment to form the native conformation is late in the folding pathway.
Large, structured RNAs traverse folding landscapes in which intermediates and long-lived misfolded states are common. To obtain a comprehensive description of the folding landscape for a structured RNA, it is necessary to understand the connections between productive folding pathways and pathways to these misfolded states. The Tetrahymena group I ribozyme partitions between folding to the nativ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2011